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February 1993

Prolonged Localized Tissue Effects From 5-Minute Exposures to Fluorouracil and Mitomycin C

Author Affiliations

From the Institute for Wound Research (Drs Khaw and Schultz) and the Department of Ophthalmology (Drs Doyle, Sherwood, and McGorray), J. Hillis Miller Health Center, University of Florida, Gainesville; and the Institute of Ophthalmology, London, England (Drs Khaw and Grierson).

Arch Ophthalmol. 1993;111(2):263-267. doi:10.1001/archopht.1993.01090020117035

• Rabbits undergoing full-thickness glaucoma filtering surgery were exposed for 5 minutes to one of three intraoperative treatments: (1) distilled water; (2) fluorouracil, 50 mg/mL; or (3) mitomycin C, 0.4 mg/mL. Tissue samples were taken from the subconjunctival and scleral tissues at the treated area and 90° and 180° from the center of the treated area and the adjacent cornea 2 mm from the limbus, 1 hour, 5 days, and 30 days postoperatively. The biopsy specimens were then placed in tissue culture media and the fibroblast outgrowths measured. Five-minute intraoperative treatments with fluorouracil resulted in a reversible delay of fibroblast outgrowths from treated subconjunctival and scleral tissues of just over 1 week in this model, whereas treatment with mitomycin C, 0.4 mg/mL, resulted in prolonged inhibition of at least 30 days. These effects were localized to the area treated. The many clinical implications of these findings are discussed.

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