We tested a new stroboscopic light source for intraoperative fluorescein angiography. A pulsed xenon light source and narrow-band interference filters are coupled to a 20-gauge endoilluminator and a 35-mm camera mounted on a surgical microscope. The system was tested with good success in a patient undergoing penetrating keratoplasty. An 18- to 22-μJ flash of 2 milliseconds' duration is achieved, which is well below the safety threshold level. This new stroboscopic light source provides a flash of sufficient light intensity to perform high-resolution, high-contrast fluorescein angiography during operations for the removal of opacified media.