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February 1995

Mitomycin C Suppresses Aqueous Humor Flow in Cynomolgus Monkeys

Author Affiliations

From the Department of Ophthalmology and Visual Sciences, Medical School, University of Wisconsin, Madison. The authors have no commercial or proprietary interest related to the subject matter in this manuscript.

Arch Ophthalmol. 1995;113(2):239-242. doi:10.1001/archopht.1995.01100020123043

Objective:  To determine whether mitomycin C suppresses aqueous humor formation in cynomolgus monkeys.

Methods:  Three monkeys received subconjunctival injections (50 μL) in four quadrants bilaterally, one eye receiving mitomycin C (0.5 mg/mL) and the other receiving distilled water. Seven monkeys underwent 360° conjunctival peritomy bilaterally and episcleral application of mitomycin C-soaked (0.5 mg/mL) cellulose sponges for 5 minutes in all four quadrants unilaterally. Aqueous humor flow was measured fluorophotometrically 1 and 3 days, and 1, 2, and 4 weeks after subconjunctival injection; and 3 days and 1, 2, 3, and 4 weeks after episcleral application.

Results:  There was no change in aqueous flow in either eye and no difference between eyes following subconjunctival injection. Aqueous flow was reduced by 8%±7% (mean±SEM), 20%±3% (P<.01), 9%±10%, and 0%±4% compared with contralateral controls 1, 2, 3, and 4 weeks, respectively, after episcleral application of mitomycin C.

Conclusions:  Episcleral application of mitomycin C can produce at least a modest short-term reduction of aqueous humor flow in primates. Reduced aqueous flow might contribute to early postoperative hypotony following trabeculectomy with mitomycin C.

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