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December 1995

Pathologic Human Vitreous Promotes Contraction by Fibroblasts: Implications for Proliferative Vitreoretinopathy

Author Affiliations

From the Department of Ophthalmology, University of Alabama at Birmingham (Drs Hardwick and Guidry), the Helen Keller Eye Research Institute, Birmingham (Drs Morris and Witherspoon), the Retina Consultants of Alabama, Birmingham (Drs White and Feist); and the Institute of Bioscience and Technology, Texas A&M University, Houston (Ms McFarland).

Arch Ophthalmol. 1995;113(12):1545-1553. doi:10.1001/archopht.1995.01100120075013

Objectives:  To establish and quantify the presence of contraction-stimulating activity in pathologic vitreous and correlate this activity with clinical presentation and outcome, especially with proliferative vitreoretinopathy.

Methods:  Contraction-stimulating activity of vitreous collected during surgery was quantified with a tissue culture assay using fibroblasts as target cells. The activity of each sample was correlated with patient history, clinical presentation, risk factors, proliferative disease, and postoperative proliferation.

Results:  Pathologic vitreous contained measurable quantities of contraction-stimulating activity and stimulated contraction in vitro, with elevated activities in samples from patients with proliferative vitreoretinopathy, epimacular proliferation, retinal detachment, retinal defects, pigmented cells in the vitreous, hemorrhage, or uveitis. Patients with postoperative proliferation had significantly elevated mean activities.

Conclusions:  Levels of contraction-stimulating activity in pathologic vitreous correlate with some risk factors for the development of proliferative vitreoretinopathy and may ultimately be useful in the assessment of disease severity and the prediction of postoperative proliferation.

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