Objective:
To analyze human corneal responses to excimer laser by immunohistochemistry.
Methods:
Corneas cultured for 3 weeks after laser ablation or taken from patients 5 to 16 months after laser treatment were exposed to antibodies to β4 integrin; types VII, IV, and III collagen; fibronectin; type I procollagen; and prolyl 4-hydroxylase. Antibody distributions were compared with those of normal corneas and unablated regions of treated corneas.
Results:
After 3 weeks, distribution of β4 integrin and types VII and IV collagen was patchy; heavy deposits of fibronectin appeared subepithelially. Keratocytes stained for prolyl 4-hydroxylase and type I procollagen. With increasing postoperative time, fibronectin diminished, and β4 integrin and type VII collagen became linear; stromal staining of types III and IV collagen increased and then diminished.
Conclusions:
Components of basement membrane, attachment complexes, and stromal matrix are synthesized shortly after laser treatment. Changes in these elements persist for 16 months in the human cornea.