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August 1996

Intravenous Infusion of Liposomal Benzoporphyrin Derivative for Photodynamic Therapy of Experimental Choroidal Neovascularization

Author Affiliations

From the Laser Research Laboratory, Retina Service, Massachusetts Eye and Ear Infirmary (Drs Husain, Miller, and Gragoudas and Mr Connolly), and the Department of Dermatology, Wellman Laboratories of Photomedicine, Massachusetts General Hospital (Mr Michaud and Dr Flotte), Harvard Medical School, Boston. The Massachusetts Eye and Ear Infirmary has a proprietary interest in this technology under a research agreement with Coherent Inc, Palo Alto, Calif, and as a part of a patent application. Drs Miller and Gragoudas are participants in this agreement and patent application under the established guidelines of Harvard Medical School.

Arch Ophthalmol. 1996;114(8):978-985. doi:10.1001/archopht.1996.01100140186012

Objective:  To compare the effectiveness of photodynamic therapy to close experimental choroidal neovascularization using an intravenous infusion of liposomal benzoporphyrin derivative (verteporfin) with previous work using a rapid intravenous injection, before initiating clinical trials.

Methods:  Choroidal neovascularization was induced in cynomolgus monkey eyes using argon laser. Liposomal benzoporphyrin derivative was delivered by an intravenous infusion pump for 10 or 32 minutes at a dose of 0.375 mg/kg. Irradiation was performed with 689- or 692-nm laser light (600-mW/cm2 irradiance and 150-J/cm2 fluence) in 7 normal eyes and 11 eyes with choroidal neovascularization between 30 and 105 minutes after the start of dye infusion. Findings were documented by fundus photography, fluorescein angiography, and light and electron microscopy.

Results:  Irradiation within 32 to 50 minutes of the start of the fast (10 minutes) or slow (32 minutes) dye infusion resulted in closure of choroidal neovascularization. In normal eyes, this technique caused choriocapillaris closure and retinal pigment epithelium damage with minimal damage to surrounding tissues.

Conclusion:  Photodynamic therapy using intravenous infusion of liposomal benzoporphyrin derivative selectively closed experimental choroidal neovascularization. This may be a suitable modality for clinical use.

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