Customize your JAMA Network experience by selecting one or more topics from the list below.
Stahl JL, Cook EB, Graziano FM, Barney NP. Human Conjunctival Mast Cells: Expression of FcϵRI, c-kit, ICAM-1, and IgE. Arch Ophthalmol. 1999;117(4):493–497. doi:https://doi.org/10.1001/archopht.117.4.493
To characterize the expression and regulation of conjunctival mast cell surface receptors important in allergic inflammation.
Mast cells were isolated from human conjunctival tissues of cadavers. Mast cell surface markers were identified using flow cytometry with antibodies to IgE, FcϵRI, c-kit, and intercellular adhesion molecule-1 (ICAM-1). We evaluated the effect of 24-hour tumor necrosis factor α (TNF-α) or interleukin 4 (IL-4) incubation on the expression of mast cell c-kit, ICAM-1, and surface-bound IgE.
Staining of mast cells (c-kit and/or tryptase positive) yielded positive results for all of the variables measured. The intensity of mast cell c-kit staining increased with TNF-α incubation, but decreased below that of unstimulated mast cells when incubated with IL-4. Anti–ICAM-1 and anti–IgE staining were increased over that of unstimulated cells when incubated with TNF-α or IL-4.
In this model, TNF-α up-regulates mast cell surface receptors and cell-bound IgE. Interleukin 4 up-regulates mast cell ICAM-1 and cell-bound IgE, but down-regulates c-kit.
Conjunctival mast cells play a critical role in the pathogenesis of atopic ocular disease. Characterization of the expression and regulation of mast cell surface receptors is important to the development of potential novel treatments for ocular inflammation.
Create a personal account or sign in to: