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Laboratory Sciences
May 2006

Basement Membrane and Collagen Deposition After Laser Subepithelial Keratomileusis and Photorefractive Keratectomy in the Leghorn Chick Eye

Author Affiliations

Author Affiliations: Massachusetts Eye and Ear Infirmary and the Schepens Eye Research Institute, Harvard Medical School, Boston, Mass.

Arch Ophthalmol. 2006;124(5):703-709. doi:10.1001/archopht.124.5.703
Abstract

Objective  To evaluate corneal scar formation and new collagen deposition after laser subepithelial keratomileusis (LASEK) compared with photorefractive keratectomy (PRK) in the leghorn chick corneal model.

Methods  Leghorn chick corneas treated with LASEK surgery (using 20% ethanol for 30 seconds) or PRK were evaluated by indirect confocal immunofluorescence and transmission electron microscopy at 1, 2, 7, 14, and 28 days after surgery. New collagen deposition was determined by dichlorotriazinylaminofluorescein staining 2 and 4 weeks after surgery.

Results  Laminin was detected around the basal layers during the immediate postoperative period and 4 weeks after LASEK surgery, and from day 2 onwards after PRK. Collagen III deposition in the cornea was about 3 times greater with PRK than with LASEK. The thickness of new collagen deposition at 4 weeks was 34 μm ± 2.5 μm in the PRK group, which was significantly greater than that of the LASEK group (11 μm ± 1 μm; P<.001).

Conclusions  Reduced subepithelial stromal tissue deposition was observed in LASEK-treated eyes compared with PRK-treated eyes. Postoperative preservation of the epithelial basement membrane and survival of epithelial cells in LASEK and possibly in epithelial laser in situ keratomileusis may contribute to this phenomenon.

Clinical Relevance  An advantage of LASEK over PRK is the reduction of postoperative haze.

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