[Skip to Content]
[Skip to Content Landing]
Views 2,146
Citations 0
Observation
August 13, 2020

Ultrastructural Evidence of Direct Viral Damage to the Olfactory Complex in Patients Testing Positive for SARS-CoV-2

Author Affiliations
  • 1Unit of Pathology, University of Pavia, Pavia, Italy
  • 2Unit of Pathology, IRCCS Policlinico S. Matteo Foundation, Pavia, Italy
  • 3Unit of Otolaringology, University of Pavia, Pavia, Italy
  • 4Unit of Otolaryngology, IRCCS Policlinico S. Matteo Foundation, Pavia, Italy
  • 5Unit of Intensive Care, University of Pavia, Pavia, Italy
  • 6Unit of Intensive Care, IRCCS Policlinico S. Matteo Foundation, Pavia, Italy
  • 7Unit of Infectious Diseases, University of Pavia, Pavia, Italy
  • 8Unit of Infectious Diseases, IRCCS Policlinico S. Matteo Foundation, Pavia, Italy
  • 9Medical Direction, IRCCS Policlinico S. Matteo Foundation, Pavia, Italy
JAMA Otolaryngol Head Neck Surg. Published online August 13, 2020. doi:10.1001/jamaoto.2020.2366

Neurological manifestations are common in patients with coronavirus disease 2019 (COVID-19), especially in those with severe disease.1 The mechanisms underlying neuromuscular damage are the objects of substantial scientific research and speculation. We report the clinicopathologic and ultrastructural postmortem findings observed in the olfactory system of 2 patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)–positive nasal swabs who underwent minimally invasive autopsy, including nasal endoscopic dissection of the olfactory complex.

Report of Cases

The first patient had anosmia and died of COVID-19 pneumonia in the intensive care unit; the second patient had ill-defined olfactory dysfunction and died of cardiopulmonary transthyretin amyloidosis. Transmission electron microscopy results revealed the presence of 80-nm to 100-nm viral particles on the cell membrane of ciliated respiratory cells in the olfactory mucosa of patient 2 (Figure 1, A). In the olfactory bulb (OB) samples of patient 1, transmission electron microscopy showed intracytoplasmic viral inclusion bodies (Figure 1, B) and interstitial viral particles (Figure 1, C). On light microscopy, the OB sections of patient 1 showed marked CD163-positive/CD68-negative microglial cell infiltration (Figure 2) that was associated with sparse CD3-positive lymphocytes, mostly of the CD8-positive cytotoxic subset. In the OB of patient 2, only rare CD163-positive microglial cells and CD3-positive/CD8-positive perivascular lymphocytes were observed. Inflammatory cell infiltration of the nasal and olfactory mucosa was more abundant in the second case, and comprised CD3-positive T lymphocytes of the helper CD4-positive and cytotoxic CD8-positive subsets, CD20-positive B lymphocytes, and CD163-positive macrophages. In the OB of the second patient, there was no ultrastructural evidence of viral particles and only rare CD163-positive microglial cells and CD3/CD8-positive perivascular lymphocytes were observed on light microscopy.

Figure 1.  Ultrastructural Findings
Ultrastructural Findings

Transmission electron microscopy micrographs showing viral particles (arrowhead) on the cytoplasmic surface (A) in the olfactory epithelium of patient 2, a viral cytoplasmic inclusion body (arrowhead) in the cytoplasm of a cell of the olfactory bulb of patient 1 (B), and viral particles in the interstitial space of the same sample from patient 1 (C).

Figure 2.  Light Microscopy and Immunohistochemical Findings From Patient 1
Light Microscopy and Immunohistochemical Findings From Patient 1

Light micrographs of the olfactory bulb sample of patient 1 showing marked hypercellularity on hematoxylin-eosin (original magnification, ×10) (A), mostly composed of CD163–positive (B)/CD68-negative (C) macrophages on immunohistochemical stains (original magnification, ×20).

Discussion

In the heterogenous spectrum of SARS-CoV-2–associated neurological manifestations,1 olfactory and gustative dysfunction has been proposed as an early and specific symptom of viral infection and can represent in some patients the only clinical manifestation of COVID-19.2,3 Whether these and other neurological manifestations are evidence of direct viral damage to the olfactory complex and central nervous system or consequences of a systemic inflammatory response has not yet been supported by in vivo observations. The recent finding of SARS-CoV-2 receptor angiotensin-converting enzyme 2 and TMPRSS2 transcripts in olfactory horizontal basal cells, microvillar cells, Bowman glands, and olfactory sustentacular cells, but not in olfactory neuron sensors, provided a proof of concept of the susceptibility of the olfactory organ to SARS-CoV-2 infection.4 We were able to identify SARS-CoV-2 particles in the OB of a patient with severe COVID-19 that were associated with a diffuse infiltration of CD163-positive macrophages and cytotoxic T lymphocytes. The presence of CD163 serves as a marker of macrophage activation induced by the proinflammatory cytokine storm in systemic inflammatory disorders, including Ebola virus infection. A possible role of CD163-positive microglia in virus-mediated inflammation and neuropathogenesis has been previously proposed in patients with HIV and HIV-related encephalitis and various degrees of neurocognitive impairment5 and might also be a result of SARS-CoV-2–induced hyperinflammation.

Although confirmatory observations on extensive patient series are needed, our findings suggest that passive diffusion and axonal transport through the olfactory complex may be a major route of SARS-CoV-2 entry into the central nervous system, as it was previously shown in animal studies with a human coronavirus strain, human coronavirus OC43.6 This report supports the clinical hypothesis that the new onset of olfactory dysfunction should either prompt immediate testing for SARS-CoV-2 infection whenever possible or might be considered an additional clinical criterion for self-isolation.

Back to top
Article Information

Corresponding Author: Patrizia Morbini MD, PhD, Unit of Pathology, University of Pavia, Via Forlanini 16, Pavia 27100, Italy (patrizia.morbini@unipv.it).

Published Online: August 13, 2020. doi:10.1001/jamaoto.2020.2366

Conflict of Interest Disclosures: Dr Morbini reported personal fees from MSD and Roche outside the submitted work. No other disclosures were reported.

References
1.
Mao  L, Jin  H, Wang  M,  et al.  Neurologic manifestations of hospitalized patients with coronavirus disease 2019 in Wuhan, China.   JAMA Neurol. 2020;77(6):1-9. doi:10.1001/jamaneurol.2020.1127PubMedGoogle ScholarCrossref
2.
Giacomelli  A, Pezzati  L, Conti  F,  et al.  Self-reported olfactory and taste disorders in SARS-CoV-2 patients: a cross-sectional study.   Clin Infect Dis. 2020;ciaa330. doi:10.1093/cid/ciaa330PubMedGoogle Scholar
3.
Lechien  JR, Hopkins  C, Saussez  S.  Letter to the editor about the Beltrán-Corbellini et al. publication: ‘acute-onset smell and taste disorders in the context of Covid-19: a pilot multicenter PCR-based case-control study’ (Eur J Neurol 2020. doi: 10.1111/ene.14273).   Eur J Neurol. 2020. doi:10.1111/ene.14357PubMedGoogle Scholar
4.
Bilinska  K, Jakubowska  P, Von Bartheld  CS, Butowt  R.  Expression of the SARS-CoV-2 entry proteins, ACE2 and TMPRSS2, in cells of the olfactory epithelium: identification of cell types and trends with age.   ACS Chem Neurosci. 2020;11(11):1555-1562. doi:10.1021/acschemneuro.0c00210PubMedGoogle ScholarCrossref
5.
Ginsberg  SD, Alldred  MJ, Gunnam  SM,  et al.  Expression profiling suggests microglial impairment in human immunodeficiency virus neuropathogenesis.   Ann Neurol. 2018;83(2):406-417. doi:10.1002/ana.25160PubMedGoogle ScholarCrossref
6.
Desforges  M, Le Coupanec  A, Dubeau  P,  et al.  Human coronaviruses and other respiratory viruses: underestimated opportunistic pathogens of the central nervous system?   Viruses. 2019;12(1):14. doi:10.3390/v12010014PubMedGoogle ScholarCrossref
×