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Poster Presentations: American Head and Neck Society
August 2006

P206 Selection of Irrigation Fluid to Eradicate Free Cancer Cells During Head and Neck Cancer Surgery

Arch Otolaryngol Head Neck Surg. 2006;132(8):904-905. doi:10.1001/archotol.132.8.904-c

Objective: To assess the growth inhibitory activity of various intraoperative irrigation fluids on free squamous cell cancer (SCC) cells in an animal wound model simulating head and neck cancer surgery.

Design: Animal study with a cancer cell–contaminated wound model and in vitro tumor growth assay.

Subjects: C3H/HeJ mice and syngeneic SCC VII cells.

Interventions: After seeding 1 million SCC VII cells at each wound, we incubated the cells in situ for 30 minutes. The wounds were irrigated for 5 minutes with 5 different fluids: distilled water, 5% Betadine Solution (povidone-iodine), 3.2% hydrogen peroxide, isotonic sodium chloride (normal saline), and cisplatin. Tumor growth and wound healing were observed. Assays using 3-(4,5-dimethylthiazol-2yl)-2,5 diphenyltetrazoliumbromide (MTT) were performed to examine tumor growth inhibition in vitro with different concentrations and exposure times of Betadine Solution and distilled water.