Ontario Cancer Institute and University Health Network, University of Toronto;
University Health Network, Toronto
Objective: To determine the global gene expression signatures of oral squamous cell carcinomas (OSCCs) and histologically normal surgical resection margins.
Design: We used the Human U133A GeneChip arrays (Affymetrix, Santa Clara, Calif), containing over 22 000 oligonucleotide sequences. Fluorescent intensities were normalized and subjected to the Binary Structured Vector Quantization method. Pathway analysis was performed using the Online Prediction Human Interaction Database to identify biologically relevant genetic networks. Deregulated gene expression was validated using quantitative real-time reverse-transcriptase polymerase chain reaction (QRT-PCR).