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Poster Presentations: American Head and Neck Society
August 2006

P239 Immunotherapy of Established Murine Squamous Cell Carcinoma Using Dendritic Cell–Tumor Fusion Hybrids

Arch Otolaryngol Head Neck Surg. 2006;132(8):912. doi:10.1001/archotol.132.8.912-b

Objective: To investigate the therapeutic efficacy of dendritic cell–tumor fusion hybrids against a murine squamous cell carcinoma (SCC)

Design: Squamous cell carcinoma VII is a poorly immunogenic murine SCC in C3H (H-2K) mice. Subdermal tumors were established by inoculation in the midabdomen of mice. Tumor diameter were measured with a Veneer caliper and used as an indication of treatment efficacy. Dendritic cells were generated from bone marrow cells, cultured with cytokines for 8 days, harvested, and mixed with cultured tumor cells in a 1:1 ratio. Cell fusion was achieved by exposing the cell mixture to an alternate electrical current to bring cells into alignment and close together, followed by a short direct electrical current pulse.

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