UCLA Medical Center, Los Angeles, Calif;
UCLA School of Dentistry, Los Angeles
Objective: Using a salivary transcriptome approach, we have already identified 7 potential human RNA biomarkers in oral cavity/oropharyngeal squamous cell carcinoma (OSCC). The objectives were to investigate whether these 7 or other gene(s) change their expression levels after surgery.
Design: RNA isolation and linear amplification from saliva, followed by expression microarray analysis and quantitative polymerase chain reaction (qPCR) validation.