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Oral Presentations: American Head and Neck Society
August 2006

S110 (−)-Gossypol, a BH3 Mimetic and Inducer of Apoptosis in Head and Neck Cancer Cells, Is Oxidized and Deactivated by ROS

Arch Otolaryngol Head Neck Surg. 2006;132(8):863. doi:10.1001/archotol.132.8.863-b

Objective: To determine the contribution of reactive oxygen species (ROS) in (−)-gossypol-induced cell death in head and neck squamous cell carcinoma (HNSCC) cell lines.

Design: The HNSCC cell lines were treated with (−)-gossypol, and generation of ROS was measured by fluorescence microscopy and flow cytometry. Cells were treated with (−)-gossypol and/or antioxidant, or with (−)-gossypol that was pretreated with hydrogen peroxide. Following treatment, generation of ROS was measured, and cell survival was assayed.