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August 1935


Author Affiliations

CHICAGO; Friedberg Fellow in Otolaryngology, Department of Otolaryngology, Rush Medical College, University of Chicago

Arch Otolaryngol. 1935;22(2):186. doi:10.1001/archotol.1935.00640030199008

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Because of its simplicity and of the good results obtained, the method of embedding temporal bones described here was deemed worth reporting. The technic was suggested by Dr. R. R. Bensley of the department of anatomy, and through his helpful guidance the practical application of the method was carried out.

The temporal bones are fixed, cut down to the desired size, decalcified and dehydrated in the usual manner. When the specimens are in equal parts of absolute alcohol and ether they are ready to be transferred to the embedding solution. This solution is made up as follows: Fifteen grams of pyroxylin (nitrocellulose) (one-half second, Hercules Powder Company, Gillespie, N. J.) is dissolved in 50 cc. of amyl acetate, making a solution of approximately 30 per cent concentration. This is diluted to about 6 per cent by the addition of 180 cc. of ether and 20 cc. of acetone.

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