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August 1965

In Vitro Culture of Osteoblasts From Otosclerotic Bone

Author Affiliations

From the Kresge Hearing Research Institute, University of Michigan.

Arch Otolaryngol. 1965;82(2):136-143. doi:10.1001/archotol.1965.00760010138012

TEN years ago, in an effort to study the physiology of fully developed otic capsule bone in a way that would allow for observation of it as a living, changing, metabolizing tissue, we undertook to transplant into tissue-culture media the small bit of bone removed as a "cupola" from the surgical dome of the horizontal canal during fenestration surgery. A considerable outgrowth of cells resulted, originating apparently from the layer of osteogenic cells lying beneath the endosteal membrane removed with the fragment of bone. The characteristics and behavior of these cells were described in a paper read before the Otosclerosis Study Group in 1955.1

None of these bone fragments appeared to have otosclerotic areas, but because they were removed from otic capsules that had otosclerosis nearby they could not, with certainty, be considered normal. However, this investigation demonstrated the feasibility of studying osteoblastic activity by tissue-culture methods and

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