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April 1966

Pitfalls in the Histochemistry of the Inner Ear

Author Affiliations

From the University of Tokyo, Department of Otorhinolaryngology.

Arch Otolaryngol. 1966;83(4):347-349. doi:10.1001/archotol.1966.00760020349012

THE CORRELATION of structure and function in the inner ear is a real challenge to the morphologist. Because of its complex anatomy, the inner ear lends itself to histochemical investigations. It is important, however, to emphasize the dangers inherent in the application of histochemical methods to the inner ear. Results of histochemical staining may depend on techniques used.1 Variations in the distribution pattern of diformazan may result from the use of fixatives. The purpose of this paper is to demonstrate these differences in the staining pattern of reduced diphosphopyridine nucleotide (DPNH) diaphorase activity.

Material and Methods  The cochleas of 25 adult albino guinea pigs were used in this study. The substrate solution was prepared by dissolving 1.5 mg of DPNH and 1.5 mg of potassium cyanide in 5 ml of 0.1 M veronal buffer at pH 7.4. Nitro blue tetrazolium was added to the substrate solution to form

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