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July 1967

Serial Section Radioautography of the Inner Ear: Histological Technique

Author Affiliations

Bethesda, Md; Boston
From the Section on Experimental Embryology, Laboratory of Neuroanatomical Sciences, National Institute of Neurological Diseases and Blindness, Bethesda, Md, and the Department of Neuropathology, Harvard Medical School, Boston. Dr. Ruben currently is at the Department of Otorhinolaryngology, New York University School of Medicine, New York.

Arch Otolaryngol. 1967;86(1):32-37. doi:10.1001/archotol.1967.00760050034007

THE FOLLOWING histological technique was developed in order to obtain routine serial sections of mouse temporal bones of a quality suitable for optimal light microscopy and radioautography. Light microscopic radioautography with tritium requires that sections be cut no thicker than 10μ and that they lie flat on the slide. These criteria are not readily met in the case of the heterogeneous tissues of the inner ear. A double embedding technique1 was discarded because too long a time was required. Paraffin embedding, used by other investigators,2-4 has resulted in poor cytological detail and artifacts in the radioautographs. Frozen sections of tissues embedded with polyvinylpyrrolidone (PVP) or gelatin were studied but the sections did not lie flat, it was difficult to obtain serial sections, and the cochlea sections usually fragmented. The method to be described is similar to one reported earlier,5 but with modifications that were found helpful in

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