This article is only available in the PDF format. Download the PDF to view the article, as well as its associated figures and tables.
The authors have great experience in techniques of cochlear preparation for electron microscopy. They present a new domain of research technique in reconstructing normal as well as experimental cochleas. Three distinct contributions are made to improve conventional histopathological technique into an up-to-date technique utilizing the phase contrast and electron microscope.
Surface Preparation Technique.—Contrasted to the conventional technique, this technique is used to demonstrate the membranous labyrinth, mainly the organ of Corti, devoid of bone. This technique is excellent for charting hair cells in early stages of pathology and forms the basis of a new cochleogram (vide infra).
Cochleogram.—Reconstruction of the organ of Corti has been developed by various authors (Guild, Schuknecht, and others), although their technique has been based on conventional histologic sections. In conventional reconstruction, only one of every ten sections is represented. This new technique offers greater accuracy.
Nerve Staining.—The authors improved the nerve staining technique of Maillet,
SAUNDERS WH. Structural Pattern of the Organ of Corti. Arch Otolaryngol. 1967;86(3):360. doi:10.1001/archotol.1967.00760050362027
Otolaryngology in JAMA: Read the Latest
Customize your JAMA Network experience by selecting one or more topics from the list below.