IN preparing histological sections using the wet celloidin technique the knife of the sliding microtone, the block being cut, and the sections cut from it must be kept wet with 80% alcohol. The block is kept wet with a steady drip from a dropping bottle. Usually the knife and the sections are kept wet by frequent applications of alcohol with a camels hair brush, which is a time-consuming procedure.
The device described here eliminates the brush procedure by providing a steady controlled flow of alcohol to the knife surface, or any part of it desired, by siphon from a small plastic bottle mounted on the knife holder.
For a sliding microtome with adjustable knife clamp, the device shown in the Figure includes: a plastic bottle, 130 ml capacity (A); a bottle holder made from an L-shaped piece of sheet aluminum bent to fit around and under the bottle—a hole in
Brown KS, Binder PA, Wakeford OS. Knife Wetting Device: Wet Celloidin Technique. Arch Otolaryngol. 1968;87(4):439–440. doi:10.1001/archotol.1968.00760060441021
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