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April 1968

Knife Wetting Device: Wet Celloidin Technique

Author Affiliations

Bethesda, Md
From the Human Genetics Branch, National Institute of Dental Research (Dr. Brown), and the Eye Research Foundation (an affiliate of the University of Maryland) (Mrs. Binder and Mr. Wakeford), Bethesda, Md.

Arch Otolaryngol. 1968;87(4):439-440. doi:10.1001/archotol.1968.00760060441021

IN preparing histological sections using the wet celloidin technique the knife of the sliding microtone, the block being cut, and the sections cut from it must be kept wet with 80% alcohol. The block is kept wet with a steady drip from a dropping bottle. Usually the knife and the sections are kept wet by frequent applications of alcohol with a camels hair brush, which is a time-consuming procedure.

The device described here eliminates the brush procedure by providing a steady controlled flow of alcohol to the knife surface, or any part of it desired, by siphon from a small plastic bottle mounted on the knife holder.

For a sliding microtome with adjustable knife clamp, the device shown in the Figure includes: a plastic bottle, 130 ml capacity (A); a bottle holder made from an L-shaped piece of sheet aluminum bent to fit around and under the bottle—a hole in

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