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September 1968

Rapid Processing of Cat Tern poral Bones

Author Affiliations

From the Otolaryngological Research Laboratory, Wayne State University, School of Medicine, Detroit.

Arch Otolaryngol. 1968;88(3):258-263. doi:10.1001/archotol.1968.00770010260009

THE LONG laborious histological procedure familiar to histological technicians is considered to be satisfactory for both human and animal temporal bones. Gross comparison of bone mass and compactness suggests the possibility of utilizing a different histological procedure for animal bones. Testing of different histological methods has resulted in a technique that is considerably shorter but displays a quality that is comparable to more standardized histological temporal bone techniques.

For decalcification, a solution of 10% formic acid and 8% hydrochloric acid, as originated by Richman et al,1 would seem favorable with respect to reduction of decalcification time. This solution was used in conjunction with electrolysis, as compared to simply elevating the solution temperature by means of external heat to the same level that is produced as a result of the passage of current when electrolysis is employed. Comparative studies by Morris and Benton2 indicated that this acid combination would

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