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May 1972

Scanning Electron Microscopy in Otorhinolaryngologic Research, 1970

Author Affiliations

St. Louis
From the Department of Otolaryngology, Washington University School of Medicine, St. Louis.

Arch Otolaryngol. 1972;95(5):477-483. doi:10.1001/archotol.1972.00770080713017

TECHNICAL sophistication and diversification have made the year since the 1969 progress report1 one of significant growth in the application of the scanning electron microscope (SEM) to otorhinolaryngologic research. A rare research opportunity now exists for the otologic investigator as structures of the inner ear are ideally suited for scanning electron microscopic study due to their diverse surface morphology and topography.

Advances in Preparative Technique  General.—Application of more sophisticated techniques for the removal of intracellular water with spatial preservation of cell membranes has led to advancements in this research area. Extensive comparative studies of air drying, drying from solvent in vacuo, low-temperature evaporation of nonfrozen solvent, and rapid freezing followed by controlled freeze drying of guinea pig tongue2-4 have been performed and comparisons of air drying and freeze drying of guinea pig organ of Corti4,5 have been made. These comparative studies provide a baseline for the