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June 1978

Scanning Electron Microscopy of the Cochlear Vasculature

Author Affiliations

From the Laboratory of Neuroanatomy, Jagiellonian University, Kraków, Poland (Dr Miodoński), the Netherlands Institute for Brain Research, Amsterdam (Dr Hodde), and the Department of Biology and Morphology, Copernicus Academy of Medicine, Kraków, Poland (Dr Kuś).

Arch Otolaryngol. 1978;104(6):313-317. doi:10.1001/archotol.1978.00790060015004

• Studies on the vascularization of the cochlea with conventional injection methods that use light microscopy have failed in viewing both the overall three-dimensional picture, as well as the detailed construction of the cochlear vascular blood vessels. This basic shortcoming can be omitted by the use of scanning electron microscopy (SEM) on corrosion casts, which only presently has been introduced.

The use of a commercial resin (Mercox) as an injected medium, followed by application of macerating fluids, provides clean and completely filled casts of the cochlear vascular system, which can then be inspected by SEM. This method not only confirms the general findings about cochlear vascularization but gives the opportunity to view the overall picture, as well as the detailed pattern of the intricate cochlear capillary beds.

(Arch Otolaryngol 104:313-317, 1978)

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