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October 1980

Evaluating the Rat Inner Ear: A Technique Using Scanning Electron Microscopy

Author Affiliations

From the Department of Toxicology and Experimental Pathology, Burroughs Wellcome Co, Research Triangle Park, NC.

Arch Otolaryngol. 1980;106(10):613-617. doi:10.1001/archotol.1980.00790340021005

• The laboratory rat is the most commonly used species in safety evaluation of pharmaceutical compounds. However, surface preparations of the organ of Corti (OC) are difficult to perform in this species largely due to problems in removing the thick, bony otic capsule. A modified technique was developed using rapid decalcification to solve this problem. In addition, scanning electron microscopy was used to quantitate hair cell damage and/or loss after exposure of the OC by microdissection. It was found that hair cell loss and damage could be easily detected and quantified in rats given gentamicin sulfate.

(Arch Otolaryngol 106:613-617, 1980)

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