• The soft-agar stem-cell assay was applied to head and neck cancer. We have successfully grown 23 (64%) of 36 head and neck tumors from both primary lesions and metastases. More poorly differentiated tumors had positive cultures more frequently than well-differentiated tumors. The plating efficiency (colonies per cells in the inoculum) averaged 0.006% (range, 0.001% to 0.08%). The system allows testing and sensitivity of individual tumors to cancericidal drugs, and our initial trials using methotrexate, bleomycin sulfate, and cisplatin (cisplatinum) show a high degree of variability between individual tumors.
(Arch Otolaryngol 106:672-674, 1980)
Mattox DE, Von Hoff DD. Culture of Human Head and Neck Cancer Stem Cells Using Soft Agar. Arch Otolaryngol. 1980;106(11):672–674. doi:10.1001/archotol.1980.00790350014005
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