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Article
November 1984

Dendritic Cell Identification in Head and Neck Lymphoid TissueNewly Recognized Cells Control T-Lymphocyte Functions

Author Affiliations

From the Department of Otolaryngology (Dr Richtsmeier) and the Department of Laboratory, Division of Research (Drs Bowers and Ellsworth, Mr Sorge, and Ms Berkowitz), The Mary Imogene Bassett Hospital, Cooperstown, NY.

Arch Otolaryngol. 1984;110(11):701-706. doi:10.1001/archotol.1984.00800370003001
Abstract

• The physiologic measurements of a subpopulation of mononuclear cells derived from head and neck lymphoid tissues are similar to those of dendritic cells are described. Dendritic cells are a subpopulation of bone marrow-derived leukocytes that were originally identified in rodents and now described in man as having central control of T-lymphocyte functions. We describe a technique for the enrichment of dendritic cells obtained from tonsils utilizing a bovine serum albumin (BSA) gradient and note that they have the light and electron microscopic appearance of dendritic cells. The measured oxidative mitogenic response and interferon-γ production in complete leukocyte cultures was compared with BSA gradient-separated preparations. The denser cells, comprised mostly of normal appearing lymphocytes, would not undergo a mitogenic response nor produce normal amounts of interferon when stimulated unless the dendritic cell-rich, lessdense fraction, was added back. The dendritic cells derived from tonsils seem to behave as a potent accessory cell for these T-lymphocyte-associated functions.

(Arch Otolaryngol 1984;110:701-706)

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