• The physiologic measurements of a subpopulation of mononuclear cells derived from head and neck lymphoid tissues are similar to those of dendritic cells are described. Dendritic cells are a subpopulation of bone marrow-derived leukocytes that were originally identified in rodents and now described in man as having central control of T-lymphocyte functions. We describe a technique for the enrichment of dendritic cells obtained from tonsils utilizing a bovine serum albumin (BSA) gradient and note that they have the light and electron microscopic appearance of dendritic cells. The measured oxidative mitogenic response and interferon-γ production in complete leukocyte cultures was compared with BSA gradient-separated preparations. The denser cells, comprised mostly of normal appearing lymphocytes, would not undergo a mitogenic response nor produce normal amounts of interferon when stimulated unless the dendritic cell-rich, lessdense fraction, was added back. The dendritic cells derived from tonsils seem to behave as a potent accessory cell for these T-lymphocyte-associated functions.
(Arch Otolaryngol 1984;110:701-706)
William J. Richtsmeier, William E. Bowers, Charles A. Ellsworth, Kevin Sorge, Mary Berkowitz. Dendritic Cell Identification in Head and Neck Lymphoid TissueNewly Recognized Cells Control T-Lymphocyte Functions. Arch Otolaryngol. 1984;110(11):701–706. doi:10.1001/archotol.1984.00800370003001