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August 1989

Adipocyte Survival After Suction and Reinjection

Author Affiliations

Houston, Tex

Arch Otolaryngol Head Neck Surg. 1989;115(8):905. doi:10.1001/archotol.1989.01860320015004

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At the American Academy of Facial Plastic and Reconstructive Surgery meeting in Washington, DC, on September 23, 1988, Richard Dolsky, MD, Havertown, Pa, presented a research study involving adipocyte survival. Dr Dolsky reviewed the pathophysiology of autograft survival. Grafts survive the first 48 to 72 hours by imbibition, followed by inosculation of new vessels from the recipient bed area into the graft. He stressed the importance of sterile technique and the importance of immobilization. His study is based on the lysosomal acid phosphatase assay. Any increase in the necrosis of adipocyte tissue has a corresponding increase in the acid phosphatase levels of the solution.

Dr Dolsky studied the injection of adipocytes through needles (15, 18, 20, 21, and 23 gauge) and noted the corresponding acid phosphatase levels (0%, 30%, 45%, 60%, and 85%), indicating more adipocyte necrosis with smaller-gauge needles and no adipocyte necrosis with the 15-gauge needle. He concluded

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