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September 1989

Alternative to Flow Cytometry for the Study of Tumor Cell Kinetics

Arch Otolaryngol Head Neck Surg. 1989;115(9):1028. doi:10.1001/archotol.1989.01860330018006

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At the 31st annual meeting of the American Society for Head and Neck Surgery held in San Francisco, Calif, April 5 and 6, 1989, Marc Coltrera, MD, and colleagues of the University of Washington, Seattle, reported on a new alternative to flow cytometry for the study of cell kinetics within tumors. They noted that earlier studies have shown a correlation between the extent of cell proliferation within a tumor and the rate of local recurrence, distant metastases, and response to radiotherapy. Using a monoclonal antibody to PCNA (Proliferating Cell Nuclear Antigen) they compared immunoperoxidase staining of fixed, paraffin-embedded archival tumor tissue with previously obtained flow cytometric data on the same tissue. Proliferating cell nuclear antigen staining was recorded on a 1 to 4 scale depending on the proportion of cells staining the specimen. In general, a good correlation was found between the two methods. Discrepancies arose

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