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May 1990

β-Tubulin Epitope Expression in Normal and Malignant Epithelial Cells

Author Affiliations

From the Departments of Otolaryngology—Head and Neck Surgery (Ms Scott and Drs Walker, Neal, Harper, and Levine), Anatomy and Cell Biology (Drs Scott and Bloodgood), Pathology (Dr Mills), and Biology (Dr Rebhun), University of Virginia Health Sciences Center, Charlottesville, and the Department of Microbiology and Immunology, Eastern Virginia Medical School, Norfolk (Dr Somers).

Arch Otolaryngol Head Neck Surg. 1990;116(5):583-589. doi:10.1001/archotol.1990.01870050083012

• The expression of a unique β-tubulin isoform (class III) was monitored in squamous cell carcinoma (SCC) and normal epithelial cells using a monoclonal tubulin antibody called TuJ1. Whole tissue homogenates of SCC, normal tissue, SCC grown in nude mice, and SCC cultured cells were examined using sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blot. TuJ1 antibody localization was performed using peroxidase immunostaining on paraffin sections of SCC, normal tissue, nude mouse SCC, and immunofluorescent microscopy of SCC cultured cells. The malignant tissues examined stained positive with TuJ1 and a general β-tubulin antibody, whereas the normal tissues stained positively only for the general β-tubulin antibody. TuJ1 epitope expression may be a useful marker for SCCs and may assist in understanding differences between normal and malignant squamous cells.

(Arch Otolaryngol Head Neck Surg. 1990;116:583-589)

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