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February 1992

The Synergistic Effects of Rhodamine 123 and Merocyanine 540: Sensitization of Human Tumor Cell Lines After Laser Exposure: A New Technique of Photodynamic Therapy

Author Affiliations

Tripler Army Medical Center Hawaii

Arch Otolaryngol Head Neck Surg. 1992;118(2):123. doi:10.1001/archotol.1992.01880020015006

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At the recent meeting of the American Academy of Otolaryngology—Head and Neck Surgery, in Kansas City, Mo, Dan J. Castro, MD, UCLA, Los Angeles, Calif, and collaborators presented their recent research regarding the synergistic effects of two photosensitizing dyes with argon laser exposure on P3 squamous carcinoma cell lines. One series of experiments dealt with the cellular uptake of rhodamine 123 and merocyanine 540 by three different leukemia and P3 squamous carcinoma cell lines. Exposure times varying from 15 to 180 minutes were used; on analysis, clear differences in uptake were demonstrated. Depending on the cell lines, the two dyes showed distinct differences in uptake. The cell lines varied in the time it took for dye saturation with merocyanine 540.

In another series of experiments, P3 tumor cells were sensitized with either a single dye or both and then exposed to the argon laser at nonthermal energy levels. When compared

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