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Article
November 1992

Chronic Ear Pathology in a Model of Neonatal Amniotic Fluid Ear Inoculation

Author Affiliations

From the Department of Otolaryngology, Massachusetts Eye and Ear Infirmary, and Department of Otology and Laryngology, Harvard Medical School, Boston, Mass (Drs Eavey and Camacho and Ms Northrop); the Department of Otorhinolaryngology, University Del Valle, Cali, Colombia (Dr Camacho); and the Department of Pathology, Hospital Nacional de Niños, San José, Costa Rica (Ms Northrop).

Arch Otolaryngol Head Neck Surg. 1992;118(11):1198-1203. doi:10.1001/archotol.1992.01880110066014
Abstract

• Human histopathologic studies have demonstrated that amniotic fluid cellular contents, keratinized squamous epithelial cells and lanugo hair, induce an intense inflammatory reaction including granulation tissue in the neonatal temporal bone. To investigate this reaction over a prolonged period, an animal model was studied. An aliquot of sterilized autologous hair and keratinized epithelial cells was placed into 14 gerbil bullae; saline was used as a contralateral control. The animals were sacrificed at intervals up to 6 months and the temporal bones were studied by light microscopy. All animals demonstrated nonpurulent inflammatory changes on the experimental side including granulation tissue, osteoneogenesis, tympanosclerosis, and cholesteatoma; the control side demonstrated minimal middle ear changes. We conclude that in this model autologous keratinized tissue provokes a foreign body response similar to the granulation tissue observed in human infants and, further, that over a prolonged period the middle ear demonstrates more severe pathologic consequences.

(Arch Otolaryngol Head Neck Surg. 1992;118:1198-1203)

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