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November 1993

Cloning Genes From an Inner Ear cDNA Library

Author Affiliations

From the Departments of Surgery/Otolaryngology (Drs Ryan, Lin, O'Driscoll, and Harris and Ms Batcher and Mr Brumm) and Neurosciences (Dr Ryan), University of California—San Diego School of Medicine and Veterans Affairs Medical Center, La Jolla, Calif.

Arch Otolaryngol Head Neck Surg. 1993;119(11):1217-1220. doi:10.1001/archotol.1993.01880230059010

A rat inner ear complementary DNA (cDNA) library containing 1.9 ×106 recombinants was constructed and evaluated. Inserts averaged 2.0 (±2.1) kilobases in length. A subset of inserts was screened for site of expression. Two cDNA transcripts were isolated based on cochlear expression restricted to the spiral ganglion. One transcript showed a high degree of homology to several long interspersed DNA elements, neuron-specific nuclear transscripts thought to be involved in gene regulation. The second transcript showed no homology to known sequences and appears to encode a neuron-specific protein of about 248 amino acids. The library can be used to identify proteins important for inner ear function and disease.

(Arch Otolaryngol Head Neck Surg. 1993;119:1217-1220)

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