A rat inner ear complementary DNA (cDNA) library containing 1.9 ×106 recombinants was constructed and evaluated. Inserts averaged 2.0 (±2.1) kilobases in length. A subset of inserts was screened for site of expression. Two cDNA transcripts were isolated based on cochlear expression restricted to the spiral ganglion. One transcript showed a high degree of homology to several long interspersed DNA elements, neuron-specific nuclear transscripts thought to be involved in gene regulation. The second transcript showed no homology to known sequences and appears to encode a neuron-specific protein of about 248 amino acids. The library can be used to identify proteins important for inner ear function and disease.
(Arch Otolaryngol Head Neck Surg. 1993;119:1217-1220)
Ryan AF, Batcher S, Lin L, Brumm D, O'Driscoll K, Harris JP. Cloning Genes From an Inner Ear cDNA Library. Arch Otolaryngol Head Neck Surg. 1993;119(11):1217–1220. doi:10.1001/archotol.1993.01880230059010
Coronavirus Resource Center
Customize your JAMA Network experience by selecting one or more topics from the list below.
Create a personal account or sign in to: