Determine total plasminogen activitor (PA) activity and urokinase-type plasminogen activator (u-PA) levels in cell-free supernatants derived from primary and metastatic squamous cell carcinoma of the head and neck.
Plasminogen activator activity was measured by spectrophotometric assay with chromogenic substrate Val-Leu-Lys-para-nitroanilide. Urokinase-type plasminogen activator levels were measured with enzyme-linked immunosorbent assay technique.
Fourteen established squamous cell carcinoma lines from patients with head and neck cancer were assayed for both total PA activity and u-PA levels at 24 to 48 hours of incubation. Compared with control and fibroblast-conditioned media, cell lines established from squamous cell carcinoma of the head and neck had significantly (P<.005) higher levels of both total PA activity and u-PA levels. Linear regression analysis showed a positive correlation (r=.65, P=.007) between total PA activity and u-PA levels.
Squamous cell carcinomas of the head and neck are able to activate plasminogen and produce u-PA in vitro. The production of PA by squamous cell carcinomas of the head and neck may play an important role in the biology of invasion and metastasis.(Arch Otolaryngol Head Neck Surg. 1994;120:989-992)
Petruzzelli GJ, Snyderman CH, Johnson JT. In Vitro Urokinase Type Plasminogen Activator Levels and Total Plasminogen Activator Activity in Squamous Cell Carcinomas of the Head and Neck. Arch Otolaryngol Head Neck Surg. 1994;120(9):989–992. doi:10.1001/archotol.1994.01880330067012
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