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April 1997

Localization of Secretory Phospholipase A2 in Mouse Cochlear Tissues and Cultured Cells

Author Affiliations

From the Departments of Anatomy and Cell Biology (Drs Zuo and Rarey), Otolaryngology (Dr Rarey), and Pediatrics (Dr Zuo), University of Florida College of Medicine, Gainesville.

Arch Otolaryngol Head Neck Surg. 1997;123(4):373-376. doi:10.1001/archotol.1997.01900040015002

Objective:  To localize the expression of secretory phospholipase A2 (sPLA2) in the cochlear duct.

Design:  By means of an immunocytochemistry technique that used rabbit anti-porcine pancreatic phospholipase A2 antiserum, sPLA2 sites were identified in vivo and in vitro in the adult mouse cochlear duct.

Subjects:  Eight C57BL/6N normal adult male mice (11-12 weeks old) (6 mice for the in vivo study, 2 mice for the in vitro study).

Results:  Different patterns of immunostaining for sPLA2 were observed in tissues of the cochlear duct. High levels of sPLA2 were detected in the following areas: the basal cells of the stria vascularis, the organ of Corti, the spiral ganglion, and the cochlear nerve; moderate levels of sPLA2 were found in the suprastrial and poststrial portions of the spiral ligament; low levels of sPLA2 were observed in the marginal and intermediate cells of the stria vascularis and the infrastrial portion of the spiral ligament. Immunoreactivity for sPLA2 also was detected in cultured marginal cells of the stria vascularis and in fibrocytes of the spiral ligament.

Conclusions:  Secretory phospholipase A2 is present in mouse cochlear tissues and individual cultured cells. These results suggest that the levels of sPLA2 expression in the cochlear duct are cell specific.Arch Otolaryngol Head Neck Surg. 1997;123:373-376