Telomerase Activity in Oral Squamous Cell Carcinoma | Cancer Biomarkers | JAMA Otolaryngology–Head & Neck Surgery | JAMA Network
[Skip to Navigation]
Access to paid content on this site is currently suspended due to excessive activity being detected from your IP address 18.206.238.77. Please contact the publisher to request reinstatement.
1.
Shaha  ARSpiro  RHShah  JPStrong  EW Squamous cell carcinoma of the floor of the mouth.  Am J Surg. 1984;148455- 459Google ScholarCrossref
2.
Franceschi  DGupta  RSpiro  RShah  JP Improved survival in the treatment of squamous cell carcinoma of the oral tongue.  Am J Surg. 1993;166360- 365Google ScholarCrossref
3.
Califano  JVan der Reit  PWestra  W Genetic progression model for head and neck cancer: implications for field cancerization.  Cancer Res. 1996;212488- 2492Google Scholar
4.
Brachman  DG Molecular biology of head and neck cancer.  Semin Oncol. 1996;21320- 329Google Scholar
5.
Kim  NWPiatyszek  MAProwse  KR  et al.  Specific association of human telomerase activity with immortal cells and cancer.  Science. 1994;2262011- 2015Google ScholarCrossref
6.
Shay  JWWerbin  HWright  W Telomeres and telomerase in human leukaemias.  Leukaemia. 1996;101255- 1261Google Scholar
7.
Counter  CMAvilion  AALeFeuvre  CE  et al.  Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity.  EMBO J. 1992;111921- 1929Google Scholar
8.
Counter  CMHirte  HWBacchetti  SHarley  CB Telomerase activity in human ovarian carcinoma.  Proc Natl Acad Sci U S A. 1994;912900- 2904Google ScholarCrossref
9.
Chadeneau  CHay  KHirte  HGallinger  SBacchetti  S Telomerase activity associated with acquisition of malignancy in human colorectal cancer.  Cancer Res. 1995;552533- 2536Google Scholar
10.
Blackburn  EH Structure and function of telomeres.  Nature. 1991;350569- 573Google ScholarCrossref
11.
Olovnikov  AM A theory of marginotomy.  J Theor Biol. 1973;41181- 190Google ScholarCrossref
12.
Harley  CB Telomere loss: mitotic clock or genetic time bomb?  Mutat Res. 1991;256271- 282Google ScholarCrossref
13.
Feng  JFunk  WDWang  SS  et al.  The RNA component of human telomerase.  Science. 1995;2691236- 1241Google ScholarCrossref
14.
Watson  JD Origin of concatemeric T7 DNA.  Nature. 1972;239197- 201Google ScholarCrossref
15.
Hiyama  KHiyama  EIshioka  S  et al.  Telomerase activity in small-cell and non-small-cell lung cancers.  J Natl Cancer Inst. 1995;87895- 902Google ScholarCrossref
16.
Hiyama  EKodama  TShinbara  K  et al.  Telomerase activity is detected in pancreatic cancer but not in benign tumors.  Cancer Res. 1997;57326- 331Google Scholar
17.
Brennan  JSMao  LHruban  R  et al.  Molecular assessment of histopathological staging in squamous-cell carcinoma of the head and neck.  N Engl J Med. 1995;332429- 435Google ScholarCrossref
18.
Slaughter  DLSouthwick  HWSmejkal  W "Field cancerization" in oral stratified squamous epithelium: clinical implications of multicentric origin.  Cancer. 1953;6963- 968Google ScholarCrossref
19.
Chung  KMukhopadhyay  TKim  T  et al.  Discordant p53 mutations in primary head and neck cancers and corresponding second primary cancers of the upper areodigestive tract.  Cancer Res. 1993;531676- 1683Google Scholar
20.
Shay  J Aging and cancer: are telomeres and telomerase the connection?  Mol Med Today. 1995;1378- 384Google ScholarCrossref
21.
Hiyama  KYoko  HKyoizumi  S  et al.  Activation of telomerase in human lymphocytes and haematopoetic progenitor cells.  J Immunol. 1995;1553711- 3715Google Scholar
22.
Yashima  KPiatyszek  MSaboorian  HM  et al.  Telomerase and in situ telomerase RNA expression in malignant and non-malignant lymph nodes.  J Clin Pathol. 1997;50110- 117Google ScholarCrossref
23.
Hiyama  EYokoyama  TTatsumoto  N  et al.  Telomerase activity in gastric cancer.  Cancer Res. 1995;553528- 3262Google Scholar
24.
Tahara  HNakanishi  TKitamoto  M  et al.  Telomerase activity in human liver tissue: comparison between chronic liver disease and hepatocellular carcinomas.  Cancer Res. 1995;552734- 2736Google Scholar
25.
Nakatani  KYoshima  NMori  H  et al.  The significant role of telomerase activity in human brain tumors.  Cancer. 1997;80471- 476Google ScholarCrossref
26.
Mutirangura  ASupiyaphun  PTrirekapan  S  et al.  Telomerase activity in oral leukoplakia and head and neck squamous cell carcinoma.  Cancer Res. 1996;563530- 3533Google Scholar
27.
Hohaus  SCavallo  SBellacosa  A  et al.  Telomerase activity in human laryngeal squamous cell carcinoma.  Clin Cancer Res. 1996;21895- 1900Google Scholar
28.
Norton  JCPiatyszek  MAWright  WEShay  JWCorey  DR Inhibition of human telomerase activity by peptide nucleic acids.  Nat Biotech. 1996;14615- 619Google ScholarCrossref
Original Article
July 1998

Telomerase Activity in Oral Squamous Cell Carcinoma

Author Affiliations

From the Departments of Otolaryngology (Drs Curran, Irish, Gullane, and Kamel-Reid) and Laboratory Medicine and Pathobiology (Drs MacMillan and Kamel-Reid), The Toronto Hospital, The University of Toronto, and The Charlie Conacher Cancer Research Laboratory, Toronto Hospital (Drs Curran, Irish, Gullane, St. Denis, MacMillan, and Kamel-Reid), The Toronto Hospital, Toronto, Ontario.

Arch Otolaryngol Head Neck Surg. 1998;124(7):784-788. doi:10.1001/archotol.124.7.784
Abstract

Background  The riboprotein telomerase has been linked to cellular immortality and is believed to play a key role in tumorigenesis.

Objective  To determine if telomerase is expressed in patients with oral squamous cell carcinoma.

Design  Twenty patient samples of oral squamous cell carcinoma and 20 adjacent histologically normal mucosal samples were assayed using the telomeric repeat amplification protocol (TRAP) method for detection of telomerase activity. The leukemic cell line, K562, was used as a positive control and the human fibroblast line, Hs21Fs, as a negative control.

Patients  Consecutive series of patients with oral squamous cell carcinoma presenting to a tertiary referral center.

Main Outcome Measure  A sample was classified as telomerase positive when an RNase-sensitive hexameric repeat ladder was observed. Absence of laddering was considered a negative result.

Results  Eighteen (90%) of 20 tumor samples and 7 (35%) of 20 adjacent histologically normal samples were telomerase positive. A statistically significant difference was observed in telomerase activity for T1 and T2 cancers compared with T4 cancers (P<.05 by analysis of variance). No statistically significant difference was observed in activity for T1 and T2 cancers vs T3 cancers.

Conclusions  The finding of telomerase activity in 90% of tumor samples is consistent with the concept of telomerase playing a key role in tumorigenesis. Further study is needed to determine the usefulness of this enzyme as a molecular marker.

×