High β-Galactosidase and Ganglioside GM1 Levels in the Human Parotid Gland | Coagulation Disorders | JAMA Otolaryngology–Head & Neck Surgery | JAMA Network
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Original Article
November 2001

High β-Galactosidase and Ganglioside GM1 Levels in the Human Parotid Gland

Author Affiliations

From the Departments of Orthodontics and Basic Sciences, School of Dentistry, University of Southern California, Los Angeles (Drs Nowroozi, Liu, and Zernik); the Department of Orthodontics, Hiroshima University School of Dentistry, Hiroshima, Japan (Dr Kawata); and the Department of Otolaryngology, University of Southern California General Hospital (Dr Rice).

Arch Otolaryngol Head Neck Surg. 2001;127(11):1381-1384. doi:10.1001/archotol.127.11.1381
Abstract

Background  Ganglioside GM1 is a membrane glycolipid typical of nerve cell membranes, where it partakes in neurotransmitter release and is catabolized by the lysosomal β-galactosidase (GM1ase) (EC 3.2.1.23). After demonstrating a novel degenerative disease of the parotid gland in mice deficient in GM1ase, mimicking the human storage disease GM1 gangliosidosis, we studied GM1ase and ganglioside GM1 content in the human parotid glands.

Study Design  Levels of GM1ase and ganglioside GM1 were determined in samples of parotid tissues and neighboring muscle (as a negative control) for 3 subjects. Tissues were also processed for histochemical demonstration of GM1ase.

Results  The mean specific activity of GM1ase was more than 6-fold higher in the healthy human parotid tissues (1.4 ± 0.5 nmol of 4-methylumbelliferone per minute per milligram of protein) relative to the neighboring muscle tissue (0.23 ± 0.07 nmol of 4-methylumbelliferone per minute per milligram of protein). Activity of GM1ase was histochemically localized mainly to striated duct and acinar cells of the parotid gland. Ganglioside GM1 content in the parotid gland was on average 30-fold higher relative to muscle.

Conclusions  Our results are consistent with previous findings reported in the mouse and the rabbit, and probably reflect a general property of the mammalian parotid glands. The novel mechanism we previously proposed for the mouse parotid saliva secretion, mimicking neurotransmitter release in ganglioside GM1–containing nerve cells, is probably applicable also to the human parotid gland. Similarly, the human parotid gland is probably also severely affected in GM1 gangliosidosis.

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