Biofilm Surface Area in the Pediatric Nasopharynx: Chronic Rhinosinusitis vs Obstructive Sleep Apnea | Infectious Diseases | JAMA Otolaryngology–Head & Neck Surgery | JAMA Network
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1.
Chan  KHAbzug  MJCoffinet  LSimoes  EAFCool  CLiu  AH Chronic rhinosinusitis in young children differs from adults: a histopathology study.  J Pediatr 2004;144206- 212PubMedGoogle Scholar
2.
Parsons  DS Chronic sinusitis: a medical or surgical disease?  Otolaryngol Clin North Am 1996;291- 9PubMedGoogle Scholar
3.
Adappa  NDCoticchia  JM Management of refractory chronic rhinosinusitis in children.  Am J Otolaryngol 2006;27384- 389PubMedGoogle Scholar
4.
Vandenberg  SJHeatley  DG Efficacy of adenoidectomy in relieving symptoms of chronic sinusitis in children.  Arch Otolaryngol Head Neck Surg 1997;123675- 678PubMedGoogle Scholar
5.
Donlan  RM Biofilms: microbial life on surfaces.  Emerg Infect Dis 2002;8881- 890PubMedGoogle Scholar
6.
Characklis  WG Attached microbial growths, II: frictional resistance due to microbial slimes.  Water Res 1973;71249- 1258Google Scholar
7.
Potera  C Forging a link between biofilms and disease.  Science 1999;2831837- 1839PubMedGoogle Scholar
8.
Lewis  K Riddle of biofilm resistance.  Antimicrob Agents Chemother 2001;45999- 1007PubMedGoogle Scholar
9.
Stewart  PSCosterton  JW Antibiotic resistance of bacteria in biofilms.  Lancet 2001;358135- 138PubMedGoogle Scholar
10.
Zuliani  GCarron  MGurrola  JColeman  CHaupert  MBerk  RCoticchia  J Identification of adenoid biofilms in chronic rhinosinusitis.  Int J Pediatr Otorhinolaryngol 2006;701613- 1617PubMedGoogle Scholar
11.
Lim  DJCoticchia  JMUeno  KHeiselman  FABakaletz  LO Glycoconjugates in the chinchilla tubotympanum.  Ann Otol Rhinol Laryngol 1991;100933- 943PubMedGoogle Scholar
12.
Ramadan  HHSanclement  JAThomas  JG Chronic rhinosinusitis and biofilms.  Otolaryngol Head Neck Surg 2005;132414- 417PubMedGoogle Scholar
13.
Costerton  JWStewart  PSGreenberg  EP Bacterial biofilms: a common cause of persistent infections.  Science 1999;2841318- 1326PubMedGoogle Scholar
14.
Palmer  RJSternberg  C Modern microscopy in biofilm research: confocal microscopy and other approaches.  Curr Opin Biotechnol 1999;10263- 268PubMedGoogle Scholar
15.
Post  JC Direct evidence of bacterial biofilms in otitis media.  Laryngoscope 2001;1112083- 2094PubMedGoogle Scholar
16.
Chole  RAFaddis  BT Evidence for microbial biofilms in tonsillar tissue.  Arch Otolaryngol Head Neck Surg 2003;129634- 636PubMedGoogle Scholar
17.
Gates  GA Adenoidectomy for otitis media with effusion.  Ann Otol Rhinol Laryngol Suppl 1994;16354- 58PubMedGoogle Scholar
18.
Maw  AR Age and adenoid size in relation to adenoidectomy in otitis media with effusion.  Am J Otolaryngol 1985;6245- 248PubMedGoogle Scholar
19.
Wolf  GCrespo  JGReis  MAM Optical and spectroscopic methods for biofilm examination and monitoring.  Rev Environ Sci Biotechnoldoi:10.1023/A: 1021238630092Google Scholar
Original Article
February 2007

Biofilm Surface Area in the Pediatric Nasopharynx: Chronic Rhinosinusitis vs Obstructive Sleep Apnea

Author Affiliations

Author Affiliations: Division of Pediatric Otolaryngology, Departments of Otolaryngology (Drs Coticchia, Zuliani, and Carron, Ms Coleman, and Mr Gurrola), and Immunology and Microbiology (Dr Berk), Wayne State University, and Department of Otolaryngology, Children's Hospital of Michigan (Dr Haupert), Detroit.

Arch Otolaryngol Head Neck Surg. 2007;133(2):110-114. doi:10.1001/archotol.133.2.110
Abstract

Objective  To compare the percentage of mucosal surface area of adenoids infected with biofilms removed from children with chronic rhinosinusitis (CRS) vs children with obstructive sleep apnea (OSA).

Design  Comparative microanatomical investigation of adenoid mucosa from patients with CRS and OSA using scanning electron microscopy.

Setting  University-affiliated hospitals and ambulatory surgery center.

Patients  Four girls and 12 boys ranging in age from 3 months to 10 years.

Main Outcome Measure  Measurements of biofilm coverage of the entire adenoidal surface.

Results  Adenoids removed from patients with CRS had dense mature biofilms covering the mucosal surface; they had a mean of 94.9% of their mucosal surface covered with mature biofilms, compared with a mean of 1.9% coverage on the adenoids removed from patients with OSA. This difference was statistically significant at P<.001.

Conclusions  Adenoids removed from patients with CRS had almost their entire mucosal surface covered with biofilms vs scant coverage for patients with OSA. Biofilms in the nasopharynx of children with CRS may act as a chronic reservoir for bacterial pathogens resistant to standard antibiotics. The mechanical debridement of the nasopharyngeal biofilms may explain the observed clinical benefit associated with adenoidectomy in this subset of pediatric patients.

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