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Original Article
October 18, 2010

Mandibular Segmental Defect Regenerated With Macroporous Biphasic Calcium Phosphate, Collagen Membrane, and Bone Marrow Graft in Dogs

Author Affiliations

Author Affiliations: Institut National de la Santé et de la Recherche Médicale (INSERM) U791, Nantes, France (Drs Jégoux, Malard, and Daculsi); BBTOCEx (Biologie et Biomatériaux du Tissu Osseux–Chirurgie Expérimentale), Nantes Veterinary School, Nantes (Drs Goyenvalle, Cognet, and Aguado); Biomatlante, Vigneux de Bretagne, France (Ms Moreau); CIC-CIT CHGU, Bordeaux, France (Dr Daculsi); and INSERM U922, Angers, France (Drs Goyenvalle and Aguado).

Arch Otolaryngol Head Neck Surg. 2010;136(10):971-978. doi:10.1001/archoto.2010.173
Abstract

Objective  To reconstruct segmental mandibulectomy using calcium phosphate ceramics and collagen membrane with a delayed bone marrow grafting in experimental animals.

Design  Defects of segmental mandibulectomy were filled with calcium phosphate granules and wrapped with a collagen membrane in 4 dogs and left empty as a control in 2 dogs. Two months later, a bone marrow graft was injected into the center of the implants. Animals were humanely killed after a 16-week delay.

Subjects  Six adult beagles were included in this study.

Intervention  Segmental mandibulectomy.

Main Outcome Measure  Bone ingrowth and material resorption in the reconstructed segment.

Results  Successful osseous colonization bridged the whole length of the defects. The good new bone formation at the center and the periosteum-like formation at the periphery suggest the osteoinductive role of the bone marrow graft and the healing scaffold role of the membrane.

Conclusions  This model succeeded in regenerating a large segmental defect in the mandible. An investigation with a postimplantation radiation delivery schedule is required with the use of this model, which should be considered as a preclinical study for a bone tissue engineering approach in patients with cancer-related bone defects.

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