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April 1997

Localization of Secretory Phospholipase A2 in Mouse Cochlear Tissues and Cultured Cells

Author Affiliations

From the Departments of Anatomy and Cell Biology (Drs Zuo and Rarey), Otolaryngology (Dr Rarey), and Pediatrics (Dr Zuo), University of Florida College of Medicine, Gainesville.

Arch Otolaryngol Head Neck Surg. 1997;123(4):373-376. doi:10.1001/archotol.1997.01900040015002

Objective:  To localize the expression of secretory phospholipase A2 (sPLA2) in the cochlear duct.

Design:  By means of an immunocytochemistry technique that used rabbit anti-porcine pancreatic phospholipase A2 antiserum, sPLA2 sites were identified in vivo and in vitro in the adult mouse cochlear duct.

Subjects:  Eight C57BL/6N normal adult male mice (11-12 weeks old) (6 mice for the in vivo study, 2 mice for the in vitro study).

Results:  Different patterns of immunostaining for sPLA2 were observed in tissues of the cochlear duct. High levels of sPLA2 were detected in the following areas: the basal cells of the stria vascularis, the organ of Corti, the spiral ganglion, and the cochlear nerve; moderate levels of sPLA2 were found in the suprastrial and poststrial portions of the spiral ligament; low levels of sPLA2 were observed in the marginal and intermediate cells of the stria vascularis and the infrastrial portion of the spiral ligament. Immunoreactivity for sPLA2 also was detected in cultured marginal cells of the stria vascularis and in fibrocytes of the spiral ligament.

Conclusions:  Secretory phospholipase A2 is present in mouse cochlear tissues and individual cultured cells. These results suggest that the levels of sPLA2 expression in the cochlear duct are cell specific.Arch Otolaryngol Head Neck Surg. 1997;123:373-376

Mayer RJ, Marshall LA.  New insights on mammalian phospholipase A2(s): comparison of arachidonoyl-selective and -nonselective enzyme .  FASEB J . 1993; 7:339-348.
Glaser KB, Mobilio D, Chang JY, Senko N.  Phospholipase A2 enzymes: regulation and inhibition .  Trends Pharmacol Sci . 1993;14:92-98.Crossref
Matsuda Y, Ogawa M, Shibata T, et al.  Distribution of immunoreactive pancreatic phospholipase A2 (IPPL-2) in various human tissues .  Res Commun Chem Pathol Pharmacol . 1995;58:281-284.
vanKuijk F, Sevanian A, Handelman GJ, Dratz EA.  A new role for phospholipase A2: protection of membranes from lipid peroxidation damage .  Trends Biochem Sci . 1987;12:31-34.Crossref
Kramer RM, Johansen B, Hession C, Pepinsky RB.  Structure and properties of a secretable phospholipase A2 from human platelets .  Adv Exp Med Biol . 1990; 275:35-45.
Pruzanski W, Vadas P.  Phospholipase A2: a mediator between proximal and distal effectors of inflammation .  Immunol Today . 1991;12:143-146.
Lee SH, Woo HW, Jung TTK, et al.  Permeability of arachidonic acid metabolites through the round window membrane in chinchillas .  Acta Otolaryngol . 1992;493:165-169.
Zuo J, Curtis LM, Yao X, ten Cate W-JF, Rarey KE.  Expression of Na,K-ATPase α and β isoforms in the neonatal rat cochlea .  Acta Otolaryngol (Stockh) . 1995; 115:497-503.Crossref
Rarey KE, Patterson K.  Establishment of inner ear epithelial cell culture: isolation, growth and characterization .  Hear Res . 1989;38:277-288.Crossref
Bar-Sagi D, Suhan JP, McCormick F, Feramisco JR.  Localization of phospholipase A2 in normal and ras-transformed cells .  J Cell Biol . 1988;106:1649-1658.Crossref
Jung TTK.  Prostaglandins, leukotrienes, and other arachidonic acid metabolites in the pathogenesis of otitis media .  Laryngoscope . 1988;98:980-993.Crossref
Jung TTK, Park YM, Miller SK, Rozehnal S, Woo HY, Baer W.  Effect of exogenous arachidonic acid metabolites applied on round window membrane on hearing and their levels in the perilymph .  Acta Otolaryngol Suppl . 1992;493: 171-176.
Ferrary E, Bernard C, Sterkers O, Escoubet B.  Prostaglandins in the semicircular canal of the frog .  Hear Res . 1987;26:139-144.Crossref
Jung TTK, Park YM, Miller S, et al.  Effect of prostaglandin, leukotriene, and arachidonic acid on experimental otitis media with effusion in chinchillas .  Ann Otol Rhinol Laryngol . 1990;99:28-32.
Satoh T, Cohen HT, Katz AI.  Different mechanisms of renal Na-K-ATPase regulation by protein kinases in proximal and distal nephron .  Am J Physiol . 1993; 265:F399-F405.
Satoh T, Cohen HT, Katz AI.  Intracellular signaling in the regulation of renal Na-K-ATPase, II: role of eicosanoids .  J Clin Invest . 1993;91:409-415.Crossref