Mucosal Wound Healing in a Rabbit Model of Subglottic Stenosis: Biochemical Analysis of Secretions | Critical Care Medicine | JAMA Otolaryngology–Head & Neck Surgery | JAMA Network
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Original Article
February 2005

Mucosal Wound Healing in a Rabbit Model of Subglottic Stenosis: Biochemical Analysis of Secretions

Arch Otolaryngol Head Neck Surg. 2005;131(2):153-157. doi:10.1001/archotol.131.2.153

Objectives  To build on work in laryngology and oral biology that suggests utility in the assay of secretions collected from wound sites as a predictive instrument to determine which infants will likely develop subglottic stenosis following endotracheal intubation and to document and describe the wound-healing process.

Design  Prospective, randomized design.

Intervention  Laser-induced subglottic injury was established in 3 rabbits. Secretions were collected from the subglottic region at 6 time points from days 4 to 21 following injury and from 4 uninjured control airways. The secretions were then subjected to enzyme-linked immunoassays for interleukin 1β and prostaglandin E2.

Subjects  Three adult New Zealand white rabbits.

Main Outcome Measures  Interleukin 1β and prostaglandin E2 levels.

Results  Measurable amounts of both mediators were obtained. Furthermore, different temporal patterns of expression were observed with interleukin 1β, showing increased levels on days 4 to 18, and with prostaglandin E2, showing increased levels on days 7 to 18. These results concur with emerging data regarding the role of each mediator in the wound-healing process.

Conclusion  Although in its infancy, the analysis of secretions collected from the site of injury in the subglottis may have utility in the management of patients following intubation-related trauma.