Effects of Antithrombogenic Agents on Microvenous Anastomoses in a Rat Model | Surgery | JAMA Otolaryngology–Head & Neck Surgery | JAMA Network
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Original Article
February 21, 2011

Effects of Antithrombogenic Agents on Microvenous Anastomoses in a Rat Model

Author Affiliations

Author Affiliations: Division of Otolaryngology, Head and Neck Surgery, Mayo Clinic, Scottsdale, Arizona. Dr Harsha is now with the Otolaryngology Service, Division of Head and Neck Oncology, Microvascular Reconstruction, Madigan Army Medical Center, Tacoma, Washington.

Arch Otolaryngol Head Neck Surg. 2011;137(2):170-174. doi:10.1001/archoto.2010.256
Abstract

Objective  To compare venous thrombosis rates among animals treated with aspirin, clopidogrel bisulfate, and ketorolac tromethamine using an anastomotic “tuck” model.

Design  Single-blind randomized animal study.

Setting  An animal laboratory at a tertiary care academic referral center.

Subjects  Forty-two retired Lewis breeder rats divided into 3 equal groups.

Interventions  Before surgical intervention, 1 group received aspirin (10 mg/kg) through gavage; 1 group, clopidogrel bisulfate (5 mg/kg) through gavage; and the final group, ketorolac tromethamine (3 mg/kg) through intramuscular injection. Each rat was then anesthetized, and the femoral veins were prepared bilaterally. A 180° venotomy was made, and the vessels were anastomosed with the tuck model set-up for anastomotic failure. The vessels were checked for patency every 15 minutes for 2 hours after clamp removal.

Main Outcome Measures  The rate of venous thrombosis and the time to thrombosis.

Results  In both the aspirin and clopidogrel groups, 2 of 28 vessels (7%) were thrombosed. Thrombosis occurred in 3 of 28 vessels (11%) in the ketorolac group (P = .86). All thromboses in the aspirin and clopidogrel groups took place at 7.5 minutes after clamp removal. In the ketorolac group, the mean time to thrombosis was 7.5 minutes (range, 0-22.5 minutes). There was no difference in time to thrombosis among the 3 groups (P = .86).

Conclusion  Using a microvenous tuck model set-up for anastomotic failure, we found no difference in the rate of thrombosis or the time to thrombosis in rats pretreated with aspirin, clopidogrel, or ketorolac.

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