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Harsha WJ, Kau RL, Kim N, Hayden RE. Effects of Antithrombogenic Agents on Microvenous Anastomoses in a Rat Model. Arch Otolaryngol Head Neck Surg. 2011;137(2):170–174. doi:10.1001/archoto.2010.256
To compare venous thrombosis rates among animals treated with aspirin, clopidogrel bisulfate, and ketorolac tromethamine using an anastomotic “tuck” model.
Single-blind randomized animal study.
An animal laboratory at a tertiary care academic referral center.
Forty-two retired Lewis breeder rats divided into 3 equal groups.
Before surgical intervention, 1 group received aspirin (10 mg/kg) through gavage; 1 group, clopidogrel bisulfate (5 mg/kg) through gavage; and the final group, ketorolac tromethamine (3 mg/kg) through intramuscular injection. Each rat was then anesthetized, and the femoral veins were prepared bilaterally. A 180° venotomy was made, and the vessels were anastomosed with the tuck model set-up for anastomotic failure. The vessels were checked for patency every 15 minutes for 2 hours after clamp removal.
Main Outcome Measures
The rate of venous thrombosis and the time to thrombosis.
In both the aspirin and clopidogrel groups, 2 of 28 vessels (7%) were thrombosed. Thrombosis occurred in 3 of 28 vessels (11%) in the ketorolac group (P = .86). All thromboses in the aspirin and clopidogrel groups took place at 7.5 minutes after clamp removal. In the ketorolac group, the mean time to thrombosis was 7.5 minutes (range, 0-22.5 minutes). There was no difference in time to thrombosis among the 3 groups (P = .86).
Using a microvenous tuck model set-up for anastomotic failure, we found no difference in the rate of thrombosis or the time to thrombosis in rats pretreated with aspirin, clopidogrel, or ketorolac.
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