One of the outstanding features of the work on excretion of bacteria by the kidney is the relationship of the technic employed to the results obtained. The use of the catheter or of methods of securing the urine other than by sterilization of the bladder wall and by direct puncture has given results that are at variance with those in which there was no instrumentation. The work of Rolly, and particularly of Biedl and Kraus, is constantly quoted when the passage of bacteria through the normal kidney is discussed. Their technic is definitely open to criticism, in that both series of experiments were carried on either with the catheter tied into the ureter, or by obtaining the urine by catheter from the bladder.
The uniformly negative results which we obtained in all of our experiments in contradistinction to those of the before-mentioned observers led us to repeat the experiments
HELMHOLZ HF, FIELD RS. THE KIDNEY: A FILTER FOR BACTERIA: III. THE RÔLE OF TECHNIC ON THE APPARENT EXCRETION OF BACTERIA BY THE KIDNEY. Am J Dis Child. 1925;29(5):641–644. doi:10.1001/archpedi.1925.04120290066009
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