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May 1930

ANTIRACHITIC EFFECT OF WINTER SUNSHINE THROUGH CELOGLASS: RESULTS OF EXPERIMENTATION ON RATS

Author Affiliations

GERMANTOWN, PA.; BOSTON
From the Infants' and Children's Hospital, and the Pediatric Departments, Harvard Medical School, Boston.

Am J Dis Child. 1930;39(5):930-934. doi:10.1001/archpedi.1930.01930170015002
Abstract

In attempting to estimate further the effectiveness of Celoglass by experimentation on rats, we utilized the general plan reported by Tisdall and Brown.1

METHOD  Every two weeks we secured twenty-five albino rats weighing between 30 and 40 Gm. These were placed at once on McCollum's rachitogenic diet 3143, and then separated into two groups. One group was put immediately in the solarium in cages within 1 foot of the Celoglass window, and the other kept in the room for rats amply guarded from possible exposure to ultraviolet rays. At the end of six weeks both groups of rats were killed by decapitation, and the blood collected and defibrinated. The inorganic phosphate was estimated at once by the method of Fiske and Subbarrow,2 and roentgenograms were taken. Following this, the percentage of ash in the bones was determined by the method of Bethke, Steenbock and Nelson,3 as outlined

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