During the last decade a number of articles have appeared on the nonprotein nitrogen constituents of the blood. The normal and pathologic limitations have been well established. With the possible exception of the albumin and globulin of the blood serum and plasma, extensive studies of the protein constituents of the blood are not commonly reported in the literature, possibly because of the difficult and time consuming methods of analysis, the absence of well defined normal values and the recognition of pathologic variations. During an investigation of the blood proteins in hypertension1 we were stimulated to extend the study to a group of children, and we report in part the results of such observations on the nitrogen distribution of the blood of children.
Five-tenths cubic centimeter of oxalated whole blood (taken by venipuncture before breakfast and after a twelve hour fast) was diluted to 10 cc. with distilled water;
BERNHARD A, LEOPOLD JS, DREKTER IJ. THE BLOOD PROTEINS OF CHILDREN: THE DISTRIBUTION OF TOTAL NITROGEN IN WHOLE BLOOD, RED BLOOD CELLS AND SERUM PROTEINS FROM THE SAME SPECIMEN. Am J Dis Child. 1934;47(6):1256–1260. doi:10.1001/archpedi.1934.01960130080003
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