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October 1934

THE BLOOD PROTEINS OF CHILDREN: II. DISTRIBUTION IN THE SAME SPECIMEN OF BLOOD OF HYDROLYZABLE, AMIDE, HUMIN, BASIC AMINO AND MONO-AMINO NITROGEN OF THE WHOLE BLOOD, RED CELLS AND SERUM PROTEINS

Am J Dis Child. 1934;48(4):819-829. doi:10.1001/archpedi.1934.01960170109009
Abstract

The distribution of the total nitrogen in whole blood, red blood cells and serum proteins of the blood in children has been reported in a previous communication.1 The present article records the results of further studies on the distribution of the amide, humin, hydrolyzable, basic amino and mono-amino nitrogen in the whole blood, red blood cells and serum proteins.

METHODS  The methods were adapted from those of Van Slyke2 and Cavett.3 The procedure for obtaining blood specimens, the separation of red blood cells and the serum proteins from the same specimen of blood and the determination of the total nitrogen have previously been described in detail.1Whole Blood.—Five-tenths cubic centimeter of oxalated blood was diluted to 10 cc. with distilled water. One cubic centimeter was removed for the determination of the total nitrogen; the remaining 9 cc. was washed into a 30 cc. Erlenmeyer flask

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