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During 1929 and 1930 morphologic studies were made of cultures obtained from the nose, throat and urine of patients with infantile paralysis. Smears of stools were likewise examined when the patients were admitted to the hospital. These steps were taken as a routine to determine whether there was anything sufficiently distinctive about the various flora to warrant further attention.
On the patient's admission to the hospital, cultures obtained from the nose and throat were planted separately on Loeffler's blood serum agar and Pelouze's medium for the gonococcus. Catheterization of the bladder was accomplished aseptically, and the urine was cultured on plain and Pelouze's agar. Specimens of the stool were obtained and smears made. All cultures were incubated for twenty-four hours; smears were then made, and all specimens taken from the cultures or stools were stained by Gram's method.Ninety-nine cultures obtained from the nose, ninety-nine from the throat, and
TOOMEY JA. MORPHOLOGIC FLORA OF THE NOSE, THROAT, STOOLS AND URINE OF PATIENTS WITH POLIOMYELITIS. Am J Dis Child. 1935;49(6):1438–1440. doi:10.1001/archpedi.1935.01970060042004
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