In the first paper1 of this series on the blood cells in early life the rationale of the study was described in some detail. It might be stated briefly as an attempt to define the variations in the number and types of blood cells which are consistent with health in infancy as distinguished from those which indicate the presence of disease. Following the first contribution, which dealt with the fluctuations in the total white cell blood count, chief emphasis has been placed on a study of the differential leukocyte count. When this phase of the investigation was started about 300 differential counts had been done already by routine methods,1 using a fixed smear on a cover-slip with Wright's stain. Before starting a more thorough and painstaking study of the different types of white cells and their fluctuations in healthy babies it seemed wise to check the results obtained
WASHBURN AH. II. A COMPARISON OF ROUTINE AND SPECIAL TECHNICS IN THE DIFFERENTIATION OF LEUKOCYTES. Am J Dis Child. 1935;50(2):395–412. doi:10.1001/archpedi.1935.01970080089008
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