The presence of the passive transfer antibody of human hypersensitivity may be readily detected by the technic of Prausnitz and Küstner.1 An appraisal of its significance in the course of allergic diseases depends, however, on the introduction of methods for quantitative determination.
Levine and Coca2 introduced two methods, which involved dilution of serum and of antigen, respectively. The first depended on the injection of progressive dilutions of serum into the skin of a normal recipient and subsequent injection of a fixed quantity of antigen into the sensitized areas. The second depended on the reaction produced by injecting progressive dilutions of antigen into areas of normal skin previously sensitized by injection of constant amounts of serum. The results of titrations with these technics were sometimes difficult to interpret because of indefinite end-points obtained on local injection of antigen.
Since it was known that the passive transfer antibody might be
LIPPARD VW, SCHMIDT WM. HUMAN PASSIVE TRANSFER ANTIBODY: I. TITRATION BY NEUTRALIZATION. Am J Dis Child. 1937;54(2):288–295. doi:10.1001/archpedi.1937.01980020082007
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