Association of Rotavirus Vaccination With the Incidence of Type 1 Diabetes in Children

Rotavirus (RV) infection has been associated with the development of type 1 diabetes (T1D) in children.¹ Rotavirus infection triggers pancreatic apoptosis in mice, and RV peptides display molecular mimicry with T-cell epitopes in pancreatic β-cell autoantigens.² We hypothesized that if natural infection with RV was a causative factor in T1D, then RV vaccination would decrease the incidence of disease over time. Therefore, using publicly available data, we examined the incidence of T1D in Australian children before and after the oral RV vaccine was introduced to the Australian National Immunisation Program in 2007.

An interrupted time-series analysis was performed on the incidence of newly diagnosed T1D in Australian children in the 8 years before compared with the 8 years after the May 2007 introduction of routine oral RV vaccination for all infants aged 6 weeks and older. National coverage for RV vaccine at this time was estimated to be 84%. Nearly all Australian children newly diagnosed with T1D are registered with the National Diabetes Services Scheme for subsidized provision of glucose testing and insulin delivery consumables (https://www.ndss.com.au/). National Diabetes Services Scheme data are provided to the Australian Institute of Health and Welfare (https://www.aihw.gov.au/). Using the publicly available data from this source, we determined the observed and modeled rates of new-onset T1D between 2000 and 2015. Population numbers for children aged 0 to 4 years, 5 to 9 years, and 10 to 14 years in Australia over this period were sourced from the Australian Bureau of Statistics website (http://www.abs.gov.au/). Interrupted time-series modeling of preintervention and postintervention patterns and changes in the numbers of newly incident cases using the published numbers of cases in children aged 0 to 14 years were performed.

This study was approved by the Royal Children's Hospital Melbourne Human Research Ethics Committee. Publicly available deidentified data were used, and therefore informed consent was not required.

Analysis was performed using Stata version 14.2 (StataCorp). Two-sided P values less than .05 were considered significant. Data analysis occurred from August 2017 to September 2018.

Between 2000 and 2015, 16 159 cases of newly diagnosed T1D in in 66 055 000 person-years among children aged 0 to 14 years were recorded by the National Diabetes Services Scheme. This equates to a mean rate of 24.4 (95% CI, 22.4-26.7) cases per 100 000 children.

In children aged 0 to 4 years, the number of incident cases of T1D decreased by 15% (rate ratio, 0.85 [95% CI, 0.75-0.97]; P = .02) after the introduction of oral RV vaccine in 2007 (Table). However, there was no evidence of a change over time in the preintervention and postintervention patterns (Figure). In children aged 5 to 9 years and 10 to 14 years, there was no change in the number of incident cases or temporal differences during the entire 16-year period.

Over past decades, the incidence of T1D has consistently increased in Australia³ and worldwide,⁴ but recent reports, including ones from Australia,⁵ indicate that the rise may be slowing or even plateauing. We report what is to our knowledge the first evidence of a decline in the incidence of T1D after the introduction of oral RV vaccine into a routine immunization schedule. This occurred in the age cohort of children born after the introduction of RV vaccine and is consistent with the hypothesis that oral RV vaccine may be protective against the development of T1D in early childhood. Ongoing surveillance will determine if the decline in incidence persists as the children advance in age.

In contrast, a Finnish population-based cohort study⁶ with a relatively small number of cases and a shorter timeframe was inconclusive regarding an association between oral RV vaccination and type 1 diabetes or celiac disease risk. It is possible that response to RV vaccination could vary by geographical location owing to genetic and environmental differences at the population level.

We report what is to our knowledge the first evidence of a decline in the incidence TID after the introduction of oral RV vaccine into a routine immunization schedule. These findings have prompted our team to do a case-control linkage study to further explore the association between RV vaccination and T1D incidence in Australian children.

Accepted for Publication: September 25, 2018.

Published Online: January 22, 2019. doi:10.1001/jamapediatrics.2018.4578

Correction: This article was corrected on July 15, 2019, to fix errors in the type 1 diabetes rate and number of incident cases reported in the Results, Table, and Figure.

Corresponding Author: Kirsten P. Perrett, MBBS, FRACP, PhD, Murdoch Children’s Research Institute, Royal Children’s Hospital, School of Population and Global Health, University of Melbourne, 50 Flemington Rd, Parkville, Victoria 3052, Australia (kirsten.perrett@rch.org.au).

Author Contributions: Ms Jachno had full access to all of the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis.

Concept and design: All authors.

Acquisition, analysis, or interpretation of data: All authors.

Drafting of the manuscript: Perrett, Jachno, Harrison.

Critical revision of the manuscript for important intellectual content: All authors.

Statistical analysis: Jachno.

Obtained funding: Perrett, Nolan, Harrison.

Administrative, technical, or material support: All authors.

Conflict of Interest Disclosures: Drs Perrett and Nolan report that their institution, Murdoch Children’s Research Institute, has received research grants from DBV Technologies, GlaxoSmithKline, Medimmune, Novartis, Novavax, Pfizer, and Seqirus. No other disclosures were reported.

Funding/Support: This work is supported by a Melbourne Children’s Clinician-Scientist Fellowship (Dr Perrett), a National Health & Medical Research Council Senior Principal Research Fellowship (grant 1080887; Dr Harrison), a Murdoch Children’s Research Institute Infection and Immunity theme grant, and a generous donation from the Colin North Diabetes Fund (Dr Harrison).

Role of the Funder/Sponsor: The funders had no role in the design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, or approval of the manuscript; and decision to submit the manuscript for publication.