In Reply Blázquez-Gamero et al have questioned 2 aspects of our report on cytomegalovirus (CMV) screening that we are happy to address. The correspondents suggest that one study limitation was our 92.9% sensitivity for saliva testing in contrast to the CHIMES study that they maintain showed 100% sensitivity for saliva testing in the report by Ross et al.1 However, the Ross et al study design did not assess saliva testing sensitivity. Ross et al1 compared the results of rapid culture and polymerase chain reaction (PCR) testing on saliva and urine specimens collected from 80 children with confirmed congenital CMV (cCMV) infection from their CHIMES screening study.2 Assay concordance was high (96%-100%) across all comparisons, but because the 80 children were initially selected based on having a CMV-positive saliva rapid culture or PCR result, high test concordance would be expected and does not assess saliva assay sensitivity. Our study performed unselected screening and compared dried blood spot (DBS) PCR with saliva PCR where we did observe infants with negative saliva and positive DBS results.3 We suggest our results reflect not only a different study methodology from the CHIMES study but also enhanced sensitivity of DBS for detection of cCMV.
Identify all potential conflicts of interest that might be relevant to your comment.
Conflicts of interest comprise financial interests, activities, and relationships within the past 3 years including but not limited to employment, affiliation, grants or funding, consultancies, honoraria or payment, speaker's bureaus, stock ownership or options, expert testimony, royalties, donation of medical equipment, or patents planned, pending, or issued.
Err on the side of full disclosure.
If you have no conflicts of interest, check "No potential conflicts of interest" in the box below. The information will be posted with your response.
Not all submitted comments are published. Please see our commenting policy for details.
Schleiss MR, McCann MT, Dollard SC. Dried Blood Spot Testing for Detection of Congenital Cytomegalovirus—Reply. JAMA Pediatr. 2021;175(8):866. doi:10.1001/jamapediatrics.2021.0758
Coronavirus Resource Center
Customize your JAMA Network experience by selecting one or more topics from the list below.